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dapi stainng solution  (Beyotime)


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    Structured Review

    Beyotime dapi stainng solution
    Dapi Stainng Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/DAPI/pm42815006-94-11-14
    Average 99 stars, based on 32584 article reviews
    dapi stainng solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Article Title: Camellia nitidissima Flower Extract Alleviates Stress-Induced Sebaceous Dysfunction by Targeting the 11β-HSD1/PI3K/Akt/mTOR Axis.
    Article Snippet: .. Briefly, cells were incubated with 100 μL of DAPI solution (Beyotime Inc., Shanghai, China) for 10 min at room temperature to stain the nuclei. .. After removing the DAPI solution, 100 μL of Nile Red solution (10 μg/mL in PBS) was added to each well and incubated at 37 ◦C in the dark for 20 min. Stained cells were imaged using a fluorescence microscope (Leica Stellaris 5, Germany), and the fluorescence intensity of Nile Red was measured at excitation and emission wavelengths of 485 nm and 565 nm, respectively.

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma.
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    TUNEL Assay:

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma.
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Staining:

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Article Title: Camellia nitidissima Flower Extract Alleviates Stress-Induced Sebaceous Dysfunction by Targeting the 11β-HSD1/PI3K/Akt/mTOR Axis.
    Article Snippet: .. Briefly, cells were incubated with 100 μL of DAPI solution (Beyotime Inc., Shanghai, China) for 10 min at room temperature to stain the nuclei. .. After removing the DAPI solution, 100 μL of Nile Red solution (10 μg/mL in PBS) was added to each well and incubated at 37 ◦C in the dark for 20 min. Stained cells were imaged using a fluorescence microscope (Leica Stellaris 5, Germany), and the fluorescence intensity of Nile Red was measured at excitation and emission wavelengths of 485 nm and 565 nm, respectively.

    Article Title: Dibutyl phthalate exposure induced hepatotoxicity via VDAC2 mediated mitochondria-dependent ferroptosis.
    Article Snippet: Widely used plasticiser, dibutyl phthalate (DBP), poses a serious threat to ecosystems and human health.. Although evidence of the toxic effects of DBP is growing, the specific mechanisms underlying its hepatotoxicity is still unclear.. Previous studies have confirmed that CH-223191 (AhR inhibitor) could not completely alleviate DBP-induced ferroptosis and the consequent hepatotoxicity, suggesting the existence of additional pathways.

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma.
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Saline:

    Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts
    Article Snippet: Nuclear staining was performed using 4’,6-diamidino-2-phenylindole (DAPI, GDP1024, Servicebio), followed by a 10-minute incubation at room temperature. .. After removing the DAPI solution, the sections were washed twice with phosphate-buffered saline (PBS, ST447-1L, Beyotime) and then mounted with glycerin. ..

    Immunofluorescence:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Confocal:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Laser-Scanning Microscopy:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Tissue:

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.

    Fluorescence:

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.

    Microscopy:

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.

    BIA-KA:

    Article Title: FAP-targeting peptide-directed nanoprobes enable tumor microenvironment-activatable MR/NIRF imaging of breast cancer primary tumor and lung metastases
    Article Snippet: Gd-DOTA was purchased from Adamas-beta Co., Ltd (Shanghai, China). .. The BCA protein concentration assay kit, enhanced CCK8 assay kit, D-Luciferin potassium salt, Lyso-Tracker, Mito-Tracker, anti-GAPDH antibody, HRP goat anti-rabbit IgG(H + L), Cy3-conjugated goat anti-rabbit IgG(H + L), and DAPI solution were all obtained from Beyotime (Shanghai, China). ..

    Protein Concentration:

    Article Title: FAP-targeting peptide-directed nanoprobes enable tumor microenvironment-activatable MR/NIRF imaging of breast cancer primary tumor and lung metastases
    Article Snippet: Gd-DOTA was purchased from Adamas-beta Co., Ltd (Shanghai, China). .. The BCA protein concentration assay kit, enhanced CCK8 assay kit, D-Luciferin potassium salt, Lyso-Tracker, Mito-Tracker, anti-GAPDH antibody, HRP goat anti-rabbit IgG(H + L), Cy3-conjugated goat anti-rabbit IgG(H + L), and DAPI solution were all obtained from Beyotime (Shanghai, China). ..

    CCK-8 Assay:

    Article Title: FAP-targeting peptide-directed nanoprobes enable tumor microenvironment-activatable MR/NIRF imaging of breast cancer primary tumor and lung metastases
    Article Snippet: Gd-DOTA was purchased from Adamas-beta Co., Ltd (Shanghai, China). .. The BCA protein concentration assay kit, enhanced CCK8 assay kit, D-Luciferin potassium salt, Lyso-Tracker, Mito-Tracker, anti-GAPDH antibody, HRP goat anti-rabbit IgG(H + L), Cy3-conjugated goat anti-rabbit IgG(H + L), and DAPI solution were all obtained from Beyotime (Shanghai, China). ..



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